Catalog No: OKEH02738 (Formerly GWB-QC6K16)
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology F13A1 ELISA Kit (Human) (OKEH02738) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F13A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F13A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F13A1 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.3% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
Anti-F13A1 Microplate96 Wells (12 x 8 Well strips)
F13A1 Lyophilized Standard2
100X Biotinylated F13A1 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Coagulation factor XIII A chain

A comparative proteomic study of plasma in Colombian childhood acute lymphoblastic leukemia. PLoS One. 14, e0221509 (2019). 31437251

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolF13A1
Gene Full Namecoagulation factor XIII A chain
Alias SymbolsbA525O21.1 (coagulation factor XIII, A1 polypeptide);coagulation factor XIII A chain;coagulation factor XIII, A polypeptide;coagulation factor XIII, A1 polypeptide;coagulation factor XIIIa;F13A;factor XIIIa;fibrin stabilizing factor, A subunit;fibrinoligase;FSF, A subunit;protein-glutamine gamma-glutamyltransferase A chain;TGase;transglutaminase A chain;transglutaminase. plasma.
NCBI Gene Id2162
Protein NameP00488
Description of TargetCoagulation factor XIII A chain
Uniprot ID
Protein Accession #NP_000120.2
Nucleotide Accession #NM_000129.3
Protein Size (# AA)732
Molecular Weight83 kDa
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