F13A1 ELISA Kit (Human) : 96 Wells (OKEH02738)

Catalog No: OKEH02738 (Formerly GWB-QC6K16)

Size:96 Wells

Human F13A1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology F13A1 ELISA Kit (Human) (OKEH02738) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F13A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F13A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F13A1 captured in well.
• ELISA Kit Range: 0.312 - 20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 3.3% (n = 20)
  Mean Inter-assay CV%: < 7.6% (n = 20)
• ELISA Kit Component:
  • Anti-F13A1 Microplate: 96 Wells (12 x 8 Well strips)
  • F13A1 Lyophilized Standard: 2
  • 100X Biotinylated F13A1 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.1 ng/mL
• Specificity: Natural and recombinant Human Coagulation factor XIII A chain
• Publications:

  A comparative proteomic study of plasma in Colombian childhood acute lymphoblastic leukemia. PLoS One. 14, e0221509 (2019). 31437251

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: F13A1
• Gene Full Name: coagulation factor XIII A chain
• Alias Symbols: F13A
• NCBI Gene Id: 2162
• Protein Name: Coagulation factor XIII A chain
• Description of Target: This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.
• Uniprot ID: P00488
• Protein Accession #: NP_000120.2
• Nucleotide Accession #: NM_000129.3
• Protein Size (# AA): 732
• Molecular Weight: 83 kDa