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Catalog No: OKCA02490
Size:96 Wells
Price: $754.00
SKU
OKCA02490
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPig
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8681-91
1:2104100-108
1:49693-99
1:88783-90
ELISA Kit PrincipleAviva Systems Biology F12 ELISA Kit (Pig) (OKCA02490) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard F12 captured in the well.
ELISA Kit Range0.625 - 40 ug/mL
ELISA Kit Recovery
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)105101-109
EDTA Plasma (n=4)8783-90
ELISA Kit ReproducibilityMean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
ELISA Kit Component
ComponentAmount
F12 Microplate96 Wells (12 x 8 Well strips)
F12 Lyophilized Standard2
100X Biotinylated F12 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.156 ug/mL
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolF12
Alias SymbolsFXII
NCBI Gene Id397474
Protein NameCoagulation factor XII
Description of TargetFactor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Uniprot IDO97507
Protein Accession #NP_999407
Nucleotide Accession #NM_214242
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