F12 ELISA Kit (Human) : 96 Wells (OKEH02737)

Catalog No: OKEH02737

Size:96T

Human F12 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology F12 ELISA Kit (Human) (OKEH02737) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F12 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F12 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-F12 Microplate: 96 Wells (12 x 8 Well strips)
  • F12 Lyophilized Standard: 2
  • 100X Biotinylated F12 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 34 pg/mL
• Specificity: Natural and recombinant Human Coagulation factor XII
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: F12
• Gene Full Name: coagulation factor XII
• Alias Symbols: beta-factor XIIa part 1;beta-factor XIIa part 2;coagulation factor XII;coagulation factor XIIa heavy chain;coagulation factor XIIa light chain;HAE3;HAEX;HAF;Hageman factor.
• NCBI Gene Id: 2161
• Protein Name: P00748
• Description of Target: Coagulation factor XII
• Uniprot ID: https://www.uniprot.org/uniprot/P00748
• Protein Accession #: NP_000496.2
• Nucleotide Accession #: NM_000505.3
• Protein Size (# AA): 615
• Molecular Weight: 68 kDa