Catalog No: OKCD07655
Size:96 Wells
Price: $750.00
SKU
OKCD07655
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology F11R ELISA Kit (Human) (OKCD07655) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for F11R has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for F11R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample F11R captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level F11R. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level F11R using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
F11R Microplate96 Wells (12 x 8 Well strips)
F11R Lyophilized Standard2
100X F11R HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.127ng/mL
Gene SymbolF11R
Gene Full NameF11 receptor
Alias SymbolsJAM, KAT, JAM1, JAMA, JCAM, CD321, PAM-1
NCBI Gene Id50848
Protein NameJunctional adhesion molecule A
Description of TargetTight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.
Swissprot IdQ9Y624
Protein Accession #NP_001335020.1
Nucleotide Accession #NM_001348091.1
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express F11R.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express F11R.
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