F11 ELISA Kit (Human) (OKCD07714)

Catalog No: OKCD07714

Size:96WELLS

F11 ELISA Kit (Human) (OKCD07714) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XI (F11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XI (F11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XI (F11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Coagulation Factor XI (F11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 46.88-3,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor XI (F11) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor XI (F11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • F11 Microplate: 96 Wells (12 x 8 Well strips)
  • F11 Lyophilized Standard: 2
  • 100X F11 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma.
• Sensitivity: < 0.227ng/mL

Target Info

• Gene Symbol: F11
• Gene Full Name: coagulation factor XI
• Alias Symbols: coagualtion factor XI;coagulation factor XI;FXI;plasma thromboplastin antecedent;PTA.
• NCBI Gene Id: 2160
• Protein Name: Coagulation factor XI
• Description of Target: Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
• Uniprot ID: P03951
• Protein Accession #: NP_000119.1
• Nucleotide Accession #: NM_000128.3