F10 Chemi-Luminescent ELISA Kit (Human) (OKCD05555)

Catalog No: OKCD05555

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor X (F10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Coagulation Factor X (F10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Coagulation Factor X (F10) level in the sample or standard.
• ELISA Kit Range: 8.23-6,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor X (F10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor X (F10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • F10 Microplate: 96 Wells (12 x 8 Well strips)
  • F10 Lyophilized Standard: 2
  • 100X F10 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 9 mL
  • Substrate Diluent: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Plasma
• Sensitivity: < 3.05pg/mL

Target Info

• Gene Symbol: F10
• Gene Full Name: coagulation factor X
• Alias Symbols: coagulation factor X;factor X;factor Xa;FX;FXA;prothrombinase;Stuart factor;Stuart-Prower factor.
• NCBI Gene Id: 2159
• Protein Name: Coagulation factor X
• Description of Target: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
• Uniprot ID: P00742
• Protein Accession #: NP_000495.1
• Nucleotide Accession #: NM_000504.3