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Catalog No: OKEH08667
Size:96T
Price: $725.00
SKU
OKEH08667
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)92-101% (n = 20)109-118% (n = 20)113-122% (n = 20)86-98% (n = 20)
EDTA plasma(n=5)90-100% (n = 20)109-119% (n = 20)83-93% (n = 20)80-90% (n = 20)
heparin plasma(n=5)92-102% (n = 20)98-109% (n = 20)96-104% (n = 20)101-111% (n = 20)
ELISA Kit PrincipleAviva Systems Biology EZH2 ELISA Kit (Human) (OKEH08667) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EZH2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EZH2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EZH2 captured in well.
ELISA Kit Range0.78 -50 ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.7% (n = 20)
Mean Inter-assay CV%: <=5.7% (n = 20)
ELISA Kit Component
ComponentAmount
EZH2 Microplate96 Wells (12 x 8 Well strips)
EZH2 Lyophilized Standard2
100X Biotinylated EZH2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
::Subcellular Location: Nucleus
::Unigene: Hs.444082
SMR: Q15910
String: 9606.ENSP00000320147
::KEGG: hsa:2146
MIM: 277590
Pfam: PF11616
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.24 ng/mL
SpecificityNatural and recombinant Human Histone-lysine N-methyltransferase EZH2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEZH2
Gene Full Nameenhancer of zeste 2 polycomb repressive complex 2 subunit
Alias Symbolsenhancer of zeste homolog 2;ENX1;ENX-1;EZH2b;histone-lysine N-methyltransferase EZH2;KMT6;KMT6A;lysine N-methyltransferase 6;WVS;WVS2.
NCBI Gene Id2146
Protein NameQ15910
Description of TargetHistone-lysine N-methyltransferase EZH2
Uniprot IDhttps://www.uniprot.org/uniprot/Q15910
Protein Accession #NP_001190176.1
Nucleotide Accession #NM_001203247.1
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