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EYA4 Antibody (OAEB00095)

100 ug
$349.00
In Stock
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Predicted Species Reactivity:
Guinea Pig, Human, Mouse, Rabbit, Zebrafish
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality:
Polyclonal
Host:
Goat
Application:
ELISA
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Gene Symbol:
EYA4
NCBI Gene Id:
2070
Protein Name:
Eyes absent homolog 4
Swissprot Id:
O95677
Protein Accession #:
NP_004091.3
Alias Symbols:
EYA4, eyes absent homolog 4 (Drosophila), HGNC:3522, DFNA10, OTTHUMP00000040267, dJ78N10.1 (eyes absent (Drosophila) homolog 4), deafness, autosomal dominant 10, eyes absent 4, RP11-704J17.4, CMD1J, DFNA10
Replacement Item:
This antibody may replace item sc-15106 from Santa Cruz Biotechnology.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express EYA4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express EYA4.
Immunogen:
This antibody is expected to recognise isoforms a, c and d (NP_004091.3, NP_742102.1 and NP_742103.1 respectively).
Predicted Homology Based on Immunogen Sequence:
Guinea Pig: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Zebrafish: 84%
Complete computational species homology data:
EYA4 Antibody (OAEB00095)
Peptide Sequence:
EDSQDLNEQSVKKTC
Datasheets/Manuals:
Printable datasheet for anti-EYA4 antibody - OAEB00095
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot: Preliminary experiments gave an approx 50kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 69.5kDa, 67.1kDa and 69.4kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Target Reference:
* Schonberger J, Wang L, Shin JT, Kim SD, Depreux FF, Zhu H, Zon L, Pizard A, Kim JB, Macrae CA, Mungall AJ, Seidman JG, Seidman CE.; Mutation in the transcriptional coactivator EYA4 causes dilated cardiomyopathy and sensorineural hearing loss.; Nat Genet. 2005 Feb 27; [Epub ahead of print]; Pubmed 15735644

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