EYA4 Antibody - C-terminal region (OAAB05922)

Catalog No: OAAB05922

Size:400UL

Western blot analysis of EYA4 Antibody (C-term) in A2058 cell lysates (35ug/lane). EYA4 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle reacted with EYA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

EYA4 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This EYA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the C-terminal region of human EYA4.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: EYA4 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: EYA4
• Alias Symbols: CMD1J, DFNA10, dJ78N10.1 (eyes absent), eyes absent homolog 4, eyes absent-like protein 4.
• NCBI Gene Id: 2070
• Protein Name: Eyes absent homolog 4|eyes absent homolog 4 isoform a [Homo sapiens]|eyes absent homolog 4 isoform b [Homo sapiens]|eyes absent homolog 4 isoform d [Homo sapiens]
• Description of Target: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye (By similarity).
• Uniprot ID: O95677
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004091.3,NP_742101.2,NP_742103.1
• Molecular Weight: 69505