EXTL3 Antibody - N-terminal region (OAAB05635)

Catalog No: OAAB05635

Size:400UL

Western blot analysis of EXTL3 Antibody (N-term) in MDA-MB435, MDA-MB468 cell lysates (35ug/lane). EXTL3 (arrow) was detected using the purified Pab.

EXTL3 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB435

EXTL3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EXTL3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

EXTL3 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IHC-P,WB
  Function: Probable glycosyltransferase (By similarity). Required for the function of REG3A in regulating keratinocyte proliferation and differentiation.
  Cellular Location: Endoplasmic reticulum membrane; Single-pass type II membrane protein
  Tissue Location Ubiquitous. Expressed in keratinocytes.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This EXTL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-72 amino acids from the N-terminal region of human EXTL3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  EXTL3 is strongly supported by BioGPS gene expression data to be expressed in MDA-MB435

• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
  
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: EXTL3 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: EXTL3
• Alias Symbols: BOTV, epididymis secretory sperm binding protein, exostoses (multiple)-like 3, exostosin tumor-like 3, exostosin-like 3, EXT-related 1, EXT-related protein 1, EXTL1L, EXTR1, glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, hereditary multiple exostoses gene isolog, ISDNA, Multiple exostosis-like protein 3, putative tumor suppressor protein EXTL3, reg receptor, REGR, RPR.
• NCBI Gene Id: 2137
• Protein Name: Exostosin-like 3|exostosin-like 3 [Homo sapiens]
• Description of Target: Glycosyltransferase which regulates the biosynthesis of heparan sulfate (HS). Important for both skeletal development and hematopoiesis, through the formation of HS proteoglycans (HSPGs) (PubMed:28132690, PubMed:28148688). Required for the function of REG3A in regulating keratinocyte proliferation and differentiation (PubMed:22727489).
• Uniprot ID: O43909
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001431.1
• Molecular Weight: 104749