EXT2 Antibody - middle region (OAAB05994)

Catalog No: OAAB05994

Size:400UL

Western blot analysis of EXT2 Antibody (Center) (Cat. #OAAB05994) in MDA-MB468 cell line lysates (35ug/lane). EXT2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of EXT2 Antibody (Center)(Cat#OAAB05994) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

EXT2 Antibody (Center) (Cat. #OAAB05994) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: FC,IF,IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-209 amino acids from the Central region of human EXT2.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50
  FC: 1:10~50
• Other Applications Image 1 Data: EXT2 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: EXT2
• Alias Symbols: AI893565, exostoses (multiple) 2, exostosin-2, glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, HS-polymerase, multiple exostoses protein 2, multiple exostoses protein 2 homolog, multiple exostosis 2, N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase, putative tumor suppressor protein EXT2, SOTV, SSMS.
• NCBI Gene Id: 14043|2132
• Protein Name: Exostosin-2|exostosin-2 isoform 1 [Homo sapiens]|exostosin-2 isoform 2 [Homo sapiens]|exostosin-2 isoform 3 [Homo sapiens]
• Description of Target: Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).|Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.
• Uniprot ID: O77783|P70428|Q93063
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000392.3,NP_001171554.1,NP_997005.1
• Molecular Weight: 82255