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Catalog No: OKCD01905 (Formerly GWB-KBBZH5)
Size:96WELLS
Price: $675.00
SKU
OKCD01905
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Datasheets/ManualsPrintable datasheet for EXT1 ELISA Kit (Human) (OKCD01905)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Exostoses 1 (EXT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Exostoses 1 (EXT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Exostoses 1 (EXT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Exostoses 1 (EXT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Exostoses 1 (EXT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Exostoses 1 (EXT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EXT1 Microplate96 Wells (12 x 8 Well Strips)
EXT1 Lyophilized Standard2 x 50 ng
100X Biotinylated EXT1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.28 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Exostoses 1 (EXT1). No significant cross-reactivity or interference between Exostoses 1 (EXT1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolEXT1
Gene Full Nameexostosin glycosyltransferase 1
Alias Symbolsexostoses (multiple) 1;exostosin-1;EXT;Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N- acetylglucosaminyltransferase;glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase;Langer-Giedion syndrome chromosome region;LGCR;LGS;multiple exostoses protein 1;N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase;putative tumor suppressor protein EXT1;TRPS2;TTV.
NCBI Gene Id2131
Protein NameExostosin-1
Description of TargetGlycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Uniprot IDQ16394
Protein Accession #NP_000118.2
Nucleotide Accession #NM_000127.2
Protein Size (# AA)746
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