EXOSC10 Protein (OPPA01354)

Catalog No: OPPA01354 (Formerly GWB-961C23)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: PM/Scl 100is supplied at a concentration of 0.8mg/ml in 20mM HEPES, pH-7.5, 100mM NaCl, 0.01mM EDTA, 0.02% SDS. Physical appearance: Sterile Filtered clear solution.
• Host: Sf9 Insect Cells
• Application: WB
  Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels).
  Product Introduction: PM/Scl 100 is a nucleolar protein and represents one crucial component of the so-called "PM/Scl complex" or "nuclear exosome", which is an RNA-processing complex consisting of a number of exoribonucleases (not to be confused with certain multivesicular bodies which are shed by cultured cells into the culture fluid and are also called exosomes).Autoantibodies against PM/Scl 100 occur in patients suffering from polymyositis, scleroderma or polymyositis/scleroderma overlap syndromes. While antibodies to other components of the exosome complex (PM/Scl 75, several Rrp proteins) can be found in some sera, practically all patients with autoantibodies against the PM/Scl complex are positive for PM/Scl 100 antibodies, making this specificity an important marker. The presence of PM/Scl 100 autoantibodies is a comparatively "good" prognostic sign in polymyositis/scleroderma overlap syndrome unlike the poor prognosis seen when other myositis and systemic sclerosis specific autoantibodies are present.
  Product Description: PM/Scl 100 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 120 kDa. PM/Scl 100 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.
• Purity: Greater than 70% as determined by SDS-PAGE.
• Application Info: Western-Blot with monoclonal anti-hexa-His-tag antibody & polymyositis patient sera.

Target Info

• Gene Symbol: PM/Scl 100
• Alias Symbols: p2, p3, p4, RRP6, PMSCL, Rrp6p, PM-Scl, PMSCL2, PM/Scl-100
• NCBI Gene Id: 5394
• Protein Name: Exosome component 10
• Description of Target: Recombinant Human PM/Scl 100
• Uniprot ID: Q01780
• Protein Accession #: NP_001001998.1
• Protein Size (# AA): Recombinant