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Catalog No: OCOA01935
Size:20UG
Price: $179.00
SKU
OCOA01935
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EXOC3 Protein Lysate (OCOA01935)

Datasheets/ManualsPrintable datasheet for EXOC3 Protein Lysate (OCOA01935)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionEXOC3 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolEXOC3
Gene Full Nameexocyst complex component 3
Alias Symbolsexocyst complex component 3;exocyst complex component Sec6;Sec 6 homolog;SEC6;SEC6L1;SEC6-like 1;Sec6p.
NCBI Gene Id11336
Protein NameA0A024QYZ6, NM_007277, NP_009208, O60645
Description of TargetExocyst complex component 3, exocyst complex component 3 [Homo sapiens], Exocyst complex component 3, isoform CRA_a, Homo sapiens exocyst complex component 3 (EXOC3), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/O60645
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_009208
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_007277
Molecular Weight85.6 kDa
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