EVPL ELISA Kit (Human) (OKCD00335)

Catalog No: OKCD00335 (Formerly GWB-KBBFR8)

Size:96WELLS

Human EVPL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	93-101%	88-95%	88-96%	90-102%
  EDTA plasma(n=5)	88-98%	97-104%	92-105%	78-92%
  heparin plasma(n=5)	84-98%	93-101%	90-98%	79-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Envoplakin (EVPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Envoplakin (EVPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Envoplakin (EVPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Envoplakin (EVPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	82-104	94
  EDTA plasma(n=5)	81-99	90
  heparin plasma(n=5)	90-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Envoplakin (EVPL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Envoplakin (EVPL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-EVPL Microplate: 96 Wells (12 x 8 Well Strips)
  • EVPL Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated EVPL Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Envoplakin (EVPL). No significant cross-reactivity or interference between Envoplakin (EVPL) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: EVPL
• Gene Full Name: envoplakin
• Alias Symbols: 210 kDa cornified envelope precursor protein;210 kDa paraneoplastic pemphigus antigen;envoplakin;EVPK;p210.
• NCBI Gene Id: 2125
• Protein Name: Envoplakin
• Description of Target: Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.
• Uniprot ID: Q92817
• Protein Accession #: NP_001307676
• Nucleotide Accession #: NM_001320747.1
• Protein Size (# AA): 2033