Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD01983 (Formerly GWB-KBBSD9)
Size:96WELLS
Price: $675.00
SKU
OKCD01983
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for ETS1 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)81-89%92-105%79-103%89-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum89-9794
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ETS1 Microplate96 Wells (12 x 8 Well Strips)
ETS1 Lyophilized Standard2 x 10 ng
100X Biotinylated ETS1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.063 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1). No significant cross-reactivity or interference between V-Ets Erythroblastosis Virus E26 Oncogene Homolog 1 (ETS1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolETS1
Gene Full NameETS proto-oncogene 1, transcription factor
Alias Symbolsp54, ETS-1, EWSR2, c-ets-1
NCBI Gene Id2113
Protein NameProtein C-ets-1
Description of TargetTranscription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
Uniprot IDP14921
Protein Accession #NP_001137292.1
Nucleotide Accession #NM_001143820.1
Protein Size (# AA)441
Write Your Own Review
You're reviewing:ETS1 ELISA Kit (Human) (OKCD01983)
Your Rating
We found other products you might like!