Ethinylestradiol ELISA Kit (OKCD02267)

Catalog No: OKCD02267

Size:96WELLS

EE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-95%	88-101%	96-105%	98-105%
  EDTA plasma(n=5)	85-92%	91-98%	87-102%	94-102%
  heparin plasma(n=5)	78-102%	98-105%	79-98%	92-99%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ethinylestradiol (EE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ethinylestradiol (EE) and unlabeled Ethinylestradiol (EE) (Standards or samples) with the pre-coated antibody specific to Ethinylestradiol (EE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ethinylestradiol (EE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ethinylestradiol (EE) in the sample.
• ELISA Kit Range: 24.69-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	93-104	101
  EDTA plasma(n=5)	86-95	92
  heparin plasma(n=5)	80-95	79

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ethinylestradiol (EE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ethinylestradiol (EE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ethinylestradiol Microplate: 96 Wells (12 x 8 Well Strips)
  • Ethinylestradiol Lyophilized Standard: 2
  • Ethinylestradiol Biotin Complex: 1
  • 100X Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Biotin Complex Diluent: 1 x 12 mL
  • Avidin/HRP Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
  • Biotin Complex Reconstitution Buffer: 1 x 300 uL
• Additional Information: CAS: 57-63-6
  PubChem: 5991
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 9.34 pg/mL
• Assay Info: Assay Methodology: Competitive Inhibition Immunoassay

Target Info

• Gene Symbol: EE
• Alias Symbols: Ethinyl estradiol; Ethynyl estradiol; Ethynylestradiol; ETHINYLESTRADIOL; Ethinyloestradiol
• Protein Name: Ethinylestradiol
• Description of Target: Ethinyl Estradiol is a semisynthetic estrogen. Ethinyl estradiol binds to the estrogen receptor complex and enters the nucleus, activating DNA transcription of genes involved in estrogenic cellular responses. This agent also inhibits 5-alpha reductase in epididymal tissue, which lowers testosterone levels and may delay progression of prostatic cancer. In addition to its antineoplastic effects, ethinyl estradiol protects against osteoporosis. In animal models, short-term therapy with this agent has been shown to provide long-term protection against breast cancer, mimicking the antitumor effects of pregnancy. (NCI04)