ETFB ELISA Kit (Human) (OKEH04676)

Catalog No: OKEH04676

Size:96T

Human ETFB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology ETFB ELISA Kit (Human) (OKEH04676) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ETFB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ETFB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ETFB captured in well.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-ETFB Microplate: 96 Wells (12 x 8 Well Strips)
  • ETFB Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • ETFB Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.12 ng/mL
• Specificity: Natural and recombinant Human Electron transfer flavoprotein subunit beta
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ETFB
• Gene Full Name: electron transfer flavoprotein subunit beta
• Alias Symbols: beta-ETF;electron transfer flavoprotein beta subunit;electron transfer flavoprotein subunit beta;electron transfer flavoprotein, beta polypeptide;electron-transferring-flavoprotein, beta polypeptide;FP585;MADD.
• NCBI Gene Id: 2109
• Protein Name: P38117
• Description of Target: Electron transfer flavoprotein subunit beta
• Uniprot ID: https://www.uniprot.org/uniprot/P38117
• Protein Accession #: NP_001014763.1
• Nucleotide Accession #: NM_001014763.1
• Protein Size (# AA): 225
• Molecular Weight: 28 KDa