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Catalog No: OKEH06636
Size:96T
Price: $625.00
SKU
OKEH06636
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Etaa1 ELISA Kit (Bovine) (OKEH06636) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Etaa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Etaa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Etaa1 captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Etaa1 Microplate96 Wells (12 x 8 Well strips)
Etaa1 Lyophilized Standard2 x 10 ng
100X Etaa1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Bovine Ewing's tumor-associated antigen 1 homolog
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolETAA1
Gene Full NameETAA1 activator of ATR kinase
Alias SymbolsETAA1, ATR kinase activator;ETAA16;Ewing tumor associated antigen 1;ewing's tumor-associated antigen 1 homolog.
NCBI Gene Id282224
Protein NameQ08DI1
Description of TargetEwing's tumor-associated antigen 1 homolog
Uniprot IDhttps://www.uniprot.org/uniprot/Q08DI1
Protein Accession #NP_001068596
Nucleotide Accession #NM_001075128
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