Catalog No: OKAU00028
Price: $0.00
SKU
OKAU00028
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Datasheets/Manuals | Printable datasheet for Estrone ELISA Kit (OKAU00028) |
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||||||||||
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ELISA Kit Linearity | Linearity was determined by taking two urine samples diluted with Assay Buffer, one with a low diluted estrone level of 71.2 pg/mL and one with a higher diluted level of 194.4 pg/mL, and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.
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ELISA Kit Principle | The DetectX® Estrone Immunoassay Kit is designed to quantitatively measure estrone present in extracted dried fecal samples, urine and tissue culture media samples. The kit is unique as it measures both non-conjugated estrone and conjugated estrone, in the form of estrone-3-sulfate and estrone 3-glucuronide, in urine and fecal samples with almost equal affinity, allowing for non-invasive testing of this steroid. Please read the complete kit insert before performing this assay. An estrone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An estrone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to estrone to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estrone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the estrone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. | ||||||||||||||||||||||||||||||
ELISA Kit Reproducibility | Intra Assay Precision
Three urine samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Estrone concentrations were:
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ELISA Kit Component |
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Additional Information | Background: Estrone, C18H22O2, also known as E1 or osterone (3-hydroxy-1,3,5(10)-estratrien-17-one) is a C-18 steroid hormone. Estrone is one of the three naturally occurring estrogens, the others being estradiol and estriol. Estrone is produced primarily from androstenedione originating from the gonads or the adrenal cortex and from estradiol by 17-hydroxysteroid dehydrogenase. Androstenedione is also converted into estrone by aromatase (CYP19) to estrone and is expressed in stromal and carcinoma or parenchymal components of breast cancer tissue. Estrone concentrations in premenopausal mammals fluctuate according to the menstrual cycle. In premenopausal women, more than 50% of the estrone is secreted by the ovaries. In prepubertal children, men and non-supplemented postmenopausal women the major portion of estrone is derived from peripheral tissue conversion of androstenedione. Interconversion of estrone and estradiol also occurs in peripheral tissue. In humans, during the follicular phase of the menstrual cycle estrone levels increase slightly. The production of estrone then increases markedly to peak at around day 13. The peak is of short duration and by day 16 the estrone levels will be low. A second peak occurs at around day 21 of the cycle and if fertilization does not occur, then the production of estrone decreases. | ||||||||||||||||||||||||||||||
:: | Detection Limit: 28.2 pg/mL | ||||||||||||||||||||||||||||||
:: | Cross Reactivity: The following cross reactants were tested in the assay and calculated at the 50% binding point.
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Reconstitution and Storage | -20°C | ||||||||||||||||||||||||||||||
Sample Type | Dried Fecal Extracts, Urine and Tissue Culture Media | ||||||||||||||||||||||||||||||
Sensitivity | 22.4 pg/mL |
Gene Full Name | Estrone |
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- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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