Estradiol ELISA Kit (Fish) (OKCA00350)

Catalog No: OKCA00350

Size:96 Wells

Product Info

• Predicted Species Reactivity: Fish
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	89%	83-95%
  Serum (n=4)	1:2	96%	91-102%
  Serum (n=4)	1:4	93%	88-97%
  Serum (n=4)	1:8	92%	85-100%

• ELISA Kit Principle: Aviva Systems Biology Estradiol ELISA Kit (Fish) (OKCA00350) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Estradiol and Horseradish Peroxidase (HRP) conjugated Estradiol. The competitive inhibition reaction is launched between with HRP labeled Estradiol and unlabeled Estradiol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Estradiol in the sample. The color development is stopped and the intensity of the color is measured.
• ELISA Kit Range: 40 pg/mL - 1400 pg/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n = 5)	0.96	90-102%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA plasma (n = 4)	0.94	89-99%

• ELISA Kit Reproducibility:

  	Mean Intra-Assay Precision %CV			Mean Inter-Assay Precision %CV
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (pg/mL)	231.441	226.99	229.933	243.898	247.123	231.441
  SD	0.034	0.033	0.03	0.039	0.038	0.038
  CV%	6.235	6.027	5.491	7.258	7.107	6.967

• ELISA Kit Component:
  • Anti-Estradiol Microplate: 96 Well (12 x 8 Well Strips)
  • Estradiol Standard: 6 x 500 uL
  • Antibody: 1 x 6 mL
  • HRP-Conjugate: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Additional Information:

  CAS#: 50-28-2

  Molecular Formula: C18H24O2

  Molecular Weight: 272.38 g/mol

• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell culture supernates and Tissue homogenates
• Sensitivity: < 25 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish E2.
  No significant cross-reactivity or interference between fish E2 and analogues
  was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Immunoassay

Target Info

• Gene Symbol: Estradiol
• Alias Symbols: E2, Estradiol, oestradiol, 17beta-estradiol
• Protein Name: Estradiol
• Description of Target: Estradiol is the 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids. Estradiol is an Estrogen. The mechanism of action of estradiol is as an Estrogen Receptor Agonist. The chemical classification of estradiol is Estradiol Congeners. Therapeutic Estradiol is a steroid sex hormone vital to the maintenance of fertility and secondary sexual characteristics in females. Typically esterified, estradiol derivatives are formulated for oral or parenteral administration. As the primary, most potent estrogen hormone produced by the ovaries, estradiol binds to and activates specific nuclear receptors. Estradiol exhibits mild anabolic and metabolic properties, and increases blood coagulability.