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Catalog No: OKCA00350
Size:96 Wells
Price: $489.00
SKU
OKCA00350
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityFish
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration< 4 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:189%83-95%
Serum (n=4)1:296%91-102%
Serum (n=4)1:493%88-97%
Serum (n=4)1:892%85-100%
ELISA Kit PrincipleAviva Systems Biology Estradiol ELISA Kit (Fish) (OKCA00350) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Estradiol and Horseradish Peroxidase (HRP) conjugated Estradiol. The competitive inhibition reaction is launched between with HRP labeled Estradiol and unlabeled Estradiol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Estradiol in the sample. The color development is stopped and the intensity of the color is measured.
ELISA Kit Range40 pg/mL - 1400 pg/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n = 5)0.9690-102%
Sample 2
Sample TypeAverage RecoveryRange
EDTA plasma (n = 4)0.9489-99%
ELISA Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020
Mean (pg/mL)231.441226.99229.933243.898247.123231.441
SD0.0340.0330.030.0390.0380.038
CV%6.2356.0275.4917.2587.1076.967
ELISA Kit Component
ComponentAmount
Anti-Estradiol Microplate96 Well (12 x 8 Well Strips)
Estradiol Standard6 x 500 uL
Antibody 1 x 6 mL
HRP-Conjugate 1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Additional Information

CAS#: 50-28-2

Molecular Formula: C18H24O2

Molecular Weight: 272.38 g/mol

Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell culture supernates and Tissue homogenates
Sensitivity< 25 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of fish E2.
No significant cross-reactivity or interference between fish E2 and analogues
was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Description
Gene SymbolEstradiol
Alias SymbolsE2, Estradiol, oestradiol, 17beta-estradiol
Protein NameEstradiol
Description of TargetEstradiol is the 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids. Estradiol is an Estrogen. The mechanism of action of estradiol is as an Estrogen Receptor Agonist. The chemical classification of estradiol is Estradiol Congeners. Therapeutic Estradiol is a steroid sex hormone vital to the maintenance of fertility and secondary sexual characteristics in females. Typically esterified, estradiol derivatives are formulated for oral or parenteral administration. As the primary, most potent estrogen hormone produced by the ovaries, estradiol binds to and activates specific nuclear receptors. Estradiol exhibits mild anabolic and metabolic properties, and increases blood coagulability.
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