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Estradiol ELISA Kit (Canine) (OKCA00245)

Catalog#: OKCA00245
More Information
Predicted Species Reactivity Canine
Kit Detection Method Colorimetric, OD450 nm
Kit Duration < 4 Hours
Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:191%89-101%
Serum (n=4)1:297%84-98%
Serum (n=4)1:485%90-101%
Serum (n=4)1:889%87-98%
Kit Principle Aviva Systems Biology Estradiol ELISA Kit (Canine) (OKCA00245) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Estradiol and Horseradish Peroxidase (HRP) conjugated Estradiol. The competitive inhibition reaction is launched between with HRP labeled Estradiol and unlabeled Estradiol with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Estradiol in the sample. The color development is stopped and the intensity of the color is measured.
Kit Range 25 pg/mL - 1,000 pg/mL
Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n = 5)0.9288-97%
Sample 2
Sample TypeAverage RecoveryRange
EDTA plasma (n = 4)0.9589-100%
Kit Reproducibility
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Mean (pg/mL)225.345240.476239.174225.345243.104230.27
Kit Component
Anti-Estradiol Microplate96 Well (12 x 8 Well Strips)
Estradiol Standard6 x 500 uL
Antibody 1 x 6 mL
HRP-Conjugate 1 x 6 mL
20X Wash Buffer1 x 15 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Additional Information

CAS#: 50-28-2

Molecular Formula: C18H24O2

Molecular Weight: 272.38 g/mol

Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue homogenates
Sensitivity < 25 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of canine E2.
No significant cross-reactivity or interference between canine E2 and analogues
was observed.
Assay Info Assay Methodology: Quantitative Competitive Immunoassay
Gene Symbol Estradiol
Alias Symbols E2, Estradiol, oestradiol, 17beta-estradiol
Protein Name Estradiol
Description of Target Estradiol is the 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids. Estradiol is an Estrogen. The mechanism of action of estradiol is as an Estrogen Receptor Agonist. The chemical classification of estradiol is Estradiol Congeners. Therapeutic Estradiol is a steroid sex hormone vital to the maintenance of fertility and secondary sexual characteristics in females. Typically esterified, estradiol derivatives are formulated for oral or parenteral administration. As the primary, most potent estrogen hormone produced by the ovaries, estradiol binds to and activates specific nuclear receptors. Estradiol exhibits mild anabolic and metabolic properties, and increases blood coagulability.
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