ESRRG ELISA Kit (Rat) (OKEH04186)

Catalog No: OKEH04186

Size:96T

Rat ESRRG ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Esrrg ELISA Kit (Rat) (OKEH04186) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Esrrg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Esrrg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Esrrg captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Component:
  • Esrrg Microplate: 96 Wells (12 x 8 Well Strips)
  • Esrrg Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Esrrg Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.177 ng/mL
• Specificity: Natural and recombinant Rat Estrogen-related receptor gamma
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Esrrg
• Gene Full Name: estrogen-related receptor gamma
• Alias Symbols: errg;estrogen receptor-related protein 3;estrogen-related receptor gamma;nuclear receptor subfamily 3 group B member 3.
• NCBI Gene Id: 360896
• Protein Name: P62510
• Description of Target: Estrogen-related receptor gamma
• Uniprot ID: https://www.uniprot.org/uniprot/P62510
• Protein Accession #: NP_976081.1
• Nucleotide Accession #: NM_203336.2
• Protein Size (# AA): 458
• Molecular Weight: 51 kDa