ESRRA Antibody (OAGA02688)

Catalog No: OAGA02688

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
OAGA02688 diluted at 1:2000

ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A: 30 ug whole cell lysate/extract of ERR alpha protein expressing 293T cells B: Control with 2.5 ug of pre-immune rabbit IgG C: Immunoprecipitation of ERR alpha by 2.5 ug of ERR alpha antibody (OAGA02688) 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (OAGA02688) diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IP, WB, CHIP
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ERR alpha. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.93mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IP: 1:100-1:500, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: ESRRA
• Gene Full Name: estrogen-related receptor alpha
• Alias Symbols: ERR1, ERRa, ESRL1, NR3B1, ERRalpha
• NCBI Gene Id: 2101
• Description of Target: The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene.
• Uniprot ID: P11474