ESR2 Antibody (OAAF01041)

Catalog No: OAAF01041 (Formerly GWB-ASC442)

Size:100 ug

Western blot analysis of extracts from HeLa cells, treated with EGF 200ng/ml 30', using Estrogen Receptor-beta Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Estrogen Receptor-beta Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-beta.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Estrogen Receptor-β (Ab-105) antibody detects endogenous levels of total Estrogen Receptor-β protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IF: 1:100~1:500; ELISA: 1:1000
• Reference: Ogawa S., Biochem. Biophys. Res. Commun. 243:122-126(1998).
  Moore J.T., Biochem. Biophys. Res. Commun. 247:75-78(1998).
  Mosselman S., FEBS Lett. 392:49-53(1996).

Target Info

• Gene Symbol: ESR2
• Gene Full Name: estrogen receptor 2
• Alias Symbols: Erb;ER-BETA;ESRB;ESR-BETA;ESTRB;estrogen receptor beta;estrogen receptor beta 4;estrogen receptor beta splice variant, ERbeta2delta7;estrogen receptor beta splice variant, ERbeta4delta7;estrogen receptor beta splice variant, ERbeta6;estrogen receptor beta splice variant, ERbeta6delta7;estrogen receptor beta splice variant, ERbeta7;estrogen receptor beta splice variant, ERbeta7delta7;estrogen receptor beta splice variant, ERbetaEx. 4L;estrogen receptor beta splice variant, ERbetaEx. 6L;NR3A2;nuclear receptor subfamily 3 group A member 2;ODG8;oestrogen receptor beta.
• NCBI Gene Id: 2100
• Protein Name: Estrogen receptor beta
• Description of Target: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
• Uniprot ID: Q92731
• Protein Accession #: NP_001035365.1
• Molecular Weight: 59 kDa