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ESR1/ER Antibody - middle region (OAAB09847)

400ul
$390.00
In Stock
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Gene Symbol:
ESR1
NCBI Gene Id:
2099
Protein Name:
Estrogen receptor
Swissprot Id:
P03372
Protein Accession #:
NP_000116.2
Alias Symbols:
ESR1, ESR, NR3A1, Estrogen receptor, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
Replacement Item:
This antibody may replace item sc-102391 from Santa Cruz Biotechnology.
Description of Target:
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Molecular Weight:
66216 Da
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB, IHC, IF
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ESR1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ESR1.
Immunogen:
This ESR1/ER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-273 amino acids from the Central region of human ESR1/ER.
Predicted Species Reactivity:
Cow, Human, Mouse, Pig, Rat
Predicted Homology Based on Immunogen Sequence:
Cow; Human; Mouse; Pig; Rat
Product Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Reconstitution and Storage:
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals:
Printable datasheet for OAAB09847
Sample Type Confirmation:

ESR1 is strongly supported by BioGPS gene expression data to be expressed in MCF7

Isotype:
Ig
Application Info:
WB: 1:1000
IHC-P: 1:10~50
IF: 1:10~50
Additional Information:
Precautions: ESR1/ER Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
:::
Function: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Isoform 3 can bind to ERE and inhibit isoform 1
:::
Cellular Location: Isoform 1: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated
Tissue Location: Widely expressed. Isoform 3 is not expressed in the pituitary gland

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