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Catalog No: OKEH02735 (Formerly GWB-BLQI5U)
Price: $600.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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ESR1 ELISA Kit (Human) : 96 Wells (OKEH02735)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02735
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ESR1 ELISA Kit (Human) (OKEH02735) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ESR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ESR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ESR1 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.2% (n = 20)
Mean Inter-assay CV%: < 7.2% (n = 20)
ELISA Kit Component
Anti-ESR1 Microplate96 Wells (12 x 8 Well strips)
ESR1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X ESR1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human Estrogen receptor
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolESR1
Gene Full Nameestrogen receptor 1
Alias SymbolsE2 receptor alpha;ER;Era;ER-alpha;ESR;ESRA;estradiol receptor;estrogen nuclear receptor alpha;estrogen receptor;estrogen receptor alpha E1-E2-1-2;estrogen receptor alpha E1-N2-E2-1-2;ESTRR;NR3A1;nuclear receptor subfamily 3 group A member 1;oestrogen receptor alpha.
NCBI Gene Id2099
Protein NameP03372
Description of TargetEstrogen receptor
Uniprot ID
Protein Accession #NP_000116.2
Nucleotide Accession #NM_000125.3
Protein Size (# AA)595
Molecular Weight66 kDa
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