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ESA Antibody (OAAD00013)

0.5 ml
$135.00
In Stock
Gene Symbol:
PON1
NCBI Gene Id:
5444
Protein Name:
Serum paraoxonase/arylesterase 1
Swissprot Id:
P27169
Alias Symbols:
ESA, PON, MVCD5,
Replacement Item:
This antibody may replace item sc-133919 from Santa Cruz Biotechnology.
Description of Target:
This antibody reacts with a 40 kD cell surface glycoprotein called epithelial specific antigen (ESA). It reacts with the same molecule which is also detected by antibodies such as 17-1A, AUA1, KS1/4, Ber-EP4, 323/A3, MOC-31, GA733, HEA125 and others.
Host:
Mouse
Clonality:
Monoclonal
Application:
IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PON1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PON1.
Immunogen:
Small Cell Lung Carcinoma Cells
Species Reactivity:
Human
Product Format:
Concentrated
Reconstitution and Storage:
Store at 2 - 8C for short term, freeze under -20C for long term storage
Datasheets/Manuals:
Printable datasheet for ESA Antibody (OAAD00013) - OAAD00013
Clone:
B29.1 (VU-ID9)
Isotype:
IgG1kappa
Application Info:
Immunohistochemistry (Paraffin/Frozen) 1:50-1:60
Additional Information:

Cellular Localization: Cell Membrane
:::

Presentation: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5.
Aliquoting Instructions: Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. GENERAL INFO: When diluting for Immunohistochemistry, ELISA, or Western Blot, make the dilution in Primary Antibody Diluting Buffer . Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Protocol Information:

Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:60 with AutoProbe III. The optimal conditions should be determined by the individual laboratory.To differentiate between mesothelioma and carcinoma.
Target Reference:
1. Tsubura, et al., J Cutan Pathol, 19: 73, 1992.
2. Szala, et al., Proc Nat Acad Sci, USA, 87: 3542-6, 1990.
Protocol:
Tips Information:

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