ESA Antibody (OAAD00013)

Catalog No: OAAD00013

Size:0.5 ml

Product Info

• Predicted Species Reactivity: Human
• Product Format: Concentrated
• Clonality: Monoclonal
• Clone: B29.1 (VU-ID9)
• Isotype: IgG1kappa
• Host: Mouse
• Application: IHC
• Additional Information:
  Cellular Localization: Cell Membrane
  
  
  Presentation: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5.
  Aliquoting Instructions: Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. GENERAL INFO: When diluting for Immunohistochemistry, ELISA, or Western Blot, make the dilution in Primary Antibody Diluting Buffer . Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
  
• Reconstitution and Storage: Store at 2 - 8C for short term, freeze under -20C for long term storage
• Immunogen: Small Cell Lung Carcinoma Cells
• Application Info: Immunohistochemistry (Paraffin/Frozen) 1:50-1:60
• Protocol Information:
  Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:60 with AutoProbe III. The optimal conditions should be determined by the individual laboratory.To differentiate between mesothelioma and carcinoma.
  
• Reference: 1. Tsubura, et al., J Cutan Pathol, 19: 73, 1992.
  2. Szala, et al., Proc Nat Acad Sci, USA, 87: 3542-6, 1990.

Target Info

• Gene Symbol: PON1
• Alias Symbols: ESA, PON, MVCD5
• NCBI Gene Id: 5444
• Protein Name: Serum paraoxonase/arylesterase 1
• Description of Target: This antibody reacts with a 40 kD cell surface glycoprotein called epithelial specific antigen (ESA). It reacts with the same molecule which is also detected by antibodies such as 17-1A, AUA1, KS1/4, Ber-EP4, 323/A3, MOC-31, GA733, HEA125 and others.
• Uniprot ID: P27169