IL6 ELISA Kit (Goat) (OKCA02061)

Catalog No: OKCA02061

Size:96 Wells

Goat IL6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Goat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	85	80-91
  Serum (n=4)	1:2	102	97-107
  Serum (n=4)	1:4	84	81-89
  Serum (n=4)	1:8	99	96-104

• ELISA Kit Principle: Aviva Systems Biology IL6 ELISA Kit (Goat) (OKCA02061) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL6 captured in well.
• ELISA Kit Range: 1.56 pg/mL-100 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	96	90-101
  EDTA plasma (n=4)	102	97-105

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-IL6 Microplate: 96 Wells (12 x 8 Well strips)
  • IL6 Lyophilized Standard: 2 x 100 pg
  • 100X Biotinylated IL6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.39 pg/mL.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL6
• Alias Symbols: IL-6
• NCBI Gene Id: 100860785
• Protein Name: Interleukin-6
• Description of Target: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation.
• Uniprot ID: Q28319
• Protein Accession #: NP_001272569
• Nucleotide Accession #: NM_001285640