Erp57 (Grp58) Antibody : HRP (OASE00105-HRP)

Catalog No: OASE00105-HRP

Size:200ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Canine, Guinea pig, Hamster, Monkey, Pig, Rabbit
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: Map.ERP57
• Isotype: IgG1
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IP, IHC
• Additional Information: Background Info: Detects ~57kDa.
  Scientific Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the
  cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding
  domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
  
  Certificate of Analysis: 0.5 ug/mL was sufficient for detection of ERp57 in 10ug of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Human Recombinant Erp57 (Grp58)
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:2000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.
  2. Williams D.B. (2005) J Cell Sci. 119: 615-623
  3. Maattanen P., et al. (2006) Biochem Cell Biol. 84: 881-889.
  4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582.
  5. Oliver J.D., et al. (1997) Science 275: 86-88.
  6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226.
  7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.
  8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305.
  9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.
  

Target Info

• Gene Symbol: PDIA3
• Gene Full Name: protein disulfide isomerase family A, member 3
• Alias Symbols: P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, PI-PLC, HsT17083, HEL-S-269, HEL-S-93n
• NCBI Gene Id: 2923
• Uniprot ID: P30101
• Protein Accession #: NP_005304.3