Size:25ul
$149.00
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OASE00362
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Erk1/2 Antibody (OASE00362)

Catalog#: OASE00362
Domestic: within 3-5 days delivery | International: 3-5 days
This product is Genway GWB-62A8C1
More Information
Predicted Species Reactivity Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus
Product Format Liquid PBS with 50% glycerol and 0.09% sodium azide
Clonality Polyclonal
Host Rabbit
Application WB, IHC, ICC, IF, FC
:: Certificate of Analysis: A 1:1000 dilution of SPC-120 was sufficient for detection for ERK1/2 in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization: Cytoplasm, Nucleus
Reconstitution and Storage Store at -20°C for up to one year. Avoid freeze/thaw cycles.
Replacement Item This antibody may replace item sc-135900 from Santa Cruz Biotechnology.
Immunogen A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Purification Peptide affinity purified
Concentration 1 mg/ml
Datasheets/Manuals Printable datasheet for OASE00362
Specificity Detects ~44kda (ERK1) and ~42kDa (ERK2)
Application Info WB: 1:1000
IHC: 1:100
lCC/IF: 1:100
FC: 1:100
Optimal dilutions should be determined by the end user.
Target Reference 1. Boulton TG. et al. (1991) Biochemistry. 30(1):278-86. 2. Yoon S., and Seger R. (2006) Growth Factors 24:21-44.
3. Wolf G. (2005) Antioxid Redox Signal 7:1337-1345.
4. Chuerland D., Marmor G., Shainskaya A. and Seger R.
(2008) J Biol Chem. Epub: //www.jbc.org/cgi/doi/10.1074/jbc.M709030200
5. Zhuang S., and Schnellmann R.G. (2006) J Pharmacol
Exp Ther 319:991-997.
Gene Symbol Mapk3
Official Gene Full Name mitogen activated protein kinase 3
NCBI Gene Id 50689
Description of Target The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. They are both located in the cytosol and mitochondria. While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases. ERK1/2 activation is generally thought to confer a survival advantage to cells; however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions. ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis.
Swissprot Id P21708
Protein Accession # NP_059043.1
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express Mapk3.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express Mapk3.
  1. What is the species homology for "Erk1/2 Antibody (OASE00362)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus".

  2. How long will it take to receive "Erk1/2 Antibody (OASE00362)"?

    This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".

  3. What buffer format is "Erk1/2 Antibody (OASE00362)" provided in?

    This item is provided in "Liquid PBS with 50% glycerol and 0.09% sodium azide".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Erk1/2 Antibody (OASE00362)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "Erk1/2 Antibody (OASE00362)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Erk1/2 Antibody (OASE00362)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Erk1/2 Antibody (OASE00362)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Erk1/2 Antibody (OASE00362)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "MAPK3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "MAPK3"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "MAPK3"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "MAPK3"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "MAPK3"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "MAPK3"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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