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Erk1/2 Antibody (OASE00362)

25ul
$149.00
In Stock
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Predicted Species Reactivity:
Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus
Product Format:
Liquid PBS with 50% glycerol and 0.09% sodium azide
Clonality:
Polyclonal
Host:
Rabbit
Application:
WB, IHC, ICC, IF, FC
:::
Certificate of Analysis: A 1:1000 dilution of SPC-120 was sufficient for detection for ERK1/2 in 20 ug of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization: Cytoplasm, Nucleus
Reconstitution and Storage:
Store at -20°C for up to one year. Avoid freeze/thaw cycles.
Gene Symbol:
Mapk3
Official Gene Full Name:
mitogen activated protein kinase 3
NCBI Gene Id:
50689
Swissprot Id:
P21708
Protein Accession #:
NP_059043.1
Replacement Item:
This antibody may replace item sc-135900 from Santa Cruz Biotechnology.
Description of Target:
The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. They are both located in the cytosol and mitochondria. While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases. ERK1/2 activation is generally thought to confer a survival advantage to cells; however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions. ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis.
Purification:
Peptide affinity purified
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Mapk3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Mapk3.
Immunogen:
A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for OASE00362
Specificity:
Detects ~44kda (ERK1) and ~42kDa (ERK2)
Application Info:
WB: 1:1000
IHC: 1:100
lCC/IF: 1:100
FC: 1:100
Optimal dilutions should be determined by the end user.
Target Reference:
1. Boulton TG. et al. (1991) Biochemistry. 30(1):278-86. 2. Yoon S., and Seger R. (2006) Growth Factors 24:21-44.
3. Wolf G. (2005) Antioxid Redox Signal 7:1337-1345.
4. Chuerland D., Marmor G., Shainskaya A. and Seger R.
(2008) J Biol Chem. Epub: //www.jbc.org/cgi/doi/10.1074/jbc.M709030200
5. Zhuang S., and Schnellmann R.G. (2006) J Pharmacol
Exp Ther 319:991-997.

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