ERK/MAPK Antibody (Bi-Phospho-Thr202/Tyr204) (OAPC00207)

Catalog No: OAPC00207 (Formerly GWB-85CE0C)

Size:100 ul

Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda -Ptase). The blot is identical to the control except that it was incubated in lambda -Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda -Ptase.

Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish
• Product Format: Liquid 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC, DB
  Quality Control Tests: Dot blots and Western blots performed with each lot.
• Reconstitution and Storage: For long-term storage, -80C is recommended, but shorter term storage at -20C is also acceptable as aliquots may be taken with freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20C.
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 and Tyr204 of rat ERK/MAPK.
• Purification: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Homology Based on Immunogen Sequence: Cow; Dog; Human; Mouse; Rat; Zebrafish
• Concentration: 0.25 mg/ml
• Specificity: Specific for the ~42k - 44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 in Western Blots of UV treated Jurkat Cells or in a rat brain lysate. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
• Application Info: DB: 1:1000
  WB: 1:1000
  IHC: 1:500
• Reference: Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 42:135-163.
  Ahn, NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127-128:201-209.
  Johnson GL, Lapadat R (2002) Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298:1911-1912.
  Tanoue TJ, Nishida, E (2003) Molecular recognitions in the MAP kinase cascades. Cellular Signaling 15:455-462.

Target Info

• Gene Symbol: MAPK1/2
• Alias Symbols: ERT1, Erk2, ERK-2, p42-MAPK
• NCBI Gene Id: 116590
• Protein Name: Mitogen-activated protein kinase 1
• Description of Target: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
• Uniprot ID: P63086
• Protein Accession #: NP_446294.1