EREG Chemi-Luminescent ELISA Kit (Human) (OKCD03439)

Catalog No: OKCD03439

Size:96WELLS

Human EREG ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-95%	85-104%	87-95%	81-102%
  EDTA Plasma (n=5)	92-99%	82-98%	94-102%	78-99%
  Heparin Plasma (n=5)	83-95%	87-95%	94-104%	90-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Epiregulin (EREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epiregulin (EREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epiregulin (EREG) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-96	92
  EDTA Plasma (n=5)	79-103	87
  Heparin Plasma (n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epiregulin (EREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epiregulin (EREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • EREG Microplate: 96 Wells (12 x 8 Well strips)
  • EREG Lyophilized Standard: 2
  • 100X EREG Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
• Sensitivity: 15.63 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EREG
• Gene Full Name: epiregulin
• Alias Symbols: Ep;EPR;ER;proepiregulin.
• NCBI Gene Id: 2069
• Protein Name: Proepiregulin
• Description of Target: Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357).
• Uniprot ID: O14944
• Protein Accession #: NP_001423
• Nucleotide Accession #: NM_001432