ERCC5 ELISA Kit (Human) (OKCD07749)

Catalog No: OKCD07749

Size:96WELLS

ERCC5 ELISA Kit (Human) (OKCD07749) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group G (XPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group G (XPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group G (XPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xeroderma Pigmentosum, Complementation Group G (XPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ERCC5 Microplate: 96 Wells (12 x 8 Well strips)
  • ERCC5 Lyophilized Standard: 2
  • 100X ERCC5 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.128ng/mL

Target Info

• Gene Symbol: ERCC5
• Gene Full Name: ERCC excision repair 5, endonuclease
• Alias Symbols: COFS3;DNA excision repair protein ERCC-5;DNA repair protein complementing XP-G cells;ERCC5-201;ERCM2;excision repair cross-complementation group 5;excision repair cross-complementing rodent repair deficiency, complementation group 5;UVDR;Xeroderma pigmentosum group G-complementing protein;xeroderma pigmentosum, complementation group G;XPG;XPGC;XPG-complementing protein.
• NCBI Gene Id: 2073
• Protein Name: DNA repair protein complementing XP-G cells
• Description of Target: Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too.
• Uniprot ID: P28715
• Protein Accession #: NP_000114.2
• Nucleotide Accession #: NM_000123.3