Catalog No: OCOA00133
Size:20UG
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Datasheets/Manuals | Printable datasheet for ERCC2 Protein Lysate (OCOA00133) |
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Predicted Species Reactivity | Homo sapiens |
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Product Format | Lyophilized |
Host | HEK293T |
Additional Information | Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate |
:: | Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
Reconstitution and Storage | The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes |
Tag | C-MYC/DDK |
Description | ERCC2 HEK293T cell transient overexpression lysate (as WB positive control) |
Gene Symbol | ERCC2 |
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Gene Full Name | ERCC excision repair 2, TFIIH core complex helicase subunit |
Alias Symbols | basic transcription factor 2 80 kDa subunit;BTF2 p80;COFS2;CXPD;DNA excision repair protein ERCC-2;DNA repair protein complementing XP-D cells;EM9;excision repair cross-complementation group 2;excision repair cross-complementing rodent repair deficiency, complementation group 2;general transcription and DNA repair factor IIH helicase subunit XPD;TFIIH;TFIIH 80 kDa subunit;TFIIH basal transcription factor complex 80 kDa subunit;TFIIH basal transcription factor complex helicase subunit;TFIIH basal transcription factor complex helicase XPB subunit;TFIIH basal transcription factor complex helicase XPD subunit;TFIIH p80;TFIIH subunit XPD;TTD;TTD1;xeroderma pigmentosum complementary group D;xeroderma pigmentosum group D-complementing protein;XPD. |
NCBI Gene Id | 2068 |
Protein Name | NM_000400, NP_000391, P18074 |
Description of Target | General transcription and DNA repair factor IIH helicase subunit XPD, general transcription and DNA repair factor IIH helicase subunit XPD isoform 1 [Homo sapiens], Homo sapiens ERCC excision repair 2, TFIIH core complex helicase subunit (ERCC2), transcript variant 1, mRNA |
Uniprot ID | https://www.uniprot.org/uniprot/P18074 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_000391 |
Nucleotide Accession # | https://www.ncbi.nlm.nih.gov/nuccore/NM_000400 |
Molecular Weight | 86.7 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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