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Datasheets/Manuals | Printable datasheet for ERCC2 Antibody (OACA07226) (OACA07226) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunohistochemistry |
Reconstitution and Storage | -20°C or -80°C |
Immunogen | Recombinant Human TFIIH basal transcription factor complex helicase XPD subunit protein (1-300AA) |
Purification | Affinity Purified |
Preservative | 0.02% sodium azide |
Formulation | PBS with, 50% glycerol, pH7.3. |
Reference | [1]"A temperature-sensitive disorder in basal transcription and DNA repair in humans."Vermeulen W., Rademakers S., Jaspers N.G.J., Appeldoorn E., Raams A., Klein B., Kleijer W.J., Hansen L.K., Hoeijmakers J.H.J.Nat. Genet. 27:299-303(2001). [2]"Two individuals with features of both xeroderma pigmentosum and trichothiodystrophy highlight the complexity of the clinical outcomes of mutations in the XPD gene."Broughton B.C., Berneburg M., Fawcett H., Taylor E.M., Arlett C.F., Nardo T., Stefanini M., Menefee E., Price V.H., Queille S., Sarasin A., Bohnert E., Krutmann J., Davidson R., Kraemer K.H., Lehmann A.R.Hum. Mol. Genet. 10:2539-2547(2001). [3]"The xeroderma pigmentosum group D (XPD) gene: one gene, two functions, three diseases."Lehmann A.R.Genes Dev. 15:15-23(2001). |
Gene Symbol | ERCC2 |
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Gene Full Name | ERCC excision repair 2, TFIIH core complex helicase subunit |
Alias Symbols | basic transcription factor 2 80 kDa subunit;BTF2 p80;COFS2;CXPD;DNA excision repair protein ERCC-2;DNA repair protein complementing XP-D cells;EM9;excision repair cross-complementation group 2;excision repair cross-complementing rodent repair deficiency, complementation group 2;general transcription and DNA repair factor IIH helicase subunit XPD;TFIIH;TFIIH 80 kDa subunit;TFIIH basal transcription factor complex 80 kDa subunit;TFIIH basal transcription factor complex helicase subunit;TFIIH basal transcription factor complex helicase XPB subunit;TFIIH basal transcription factor complex helicase XPD subunit;TFIIH p80;TFIIH subunit XPD;TTD;TTD1;xeroderma pigmentosum complementary group D;xeroderma pigmentosum group D-complementing protein;XPD. |
NCBI Gene Id | 2068 |
Protein Name | General transcription and DNA repair factor IIH helicase subunit XPD |
Description of Target | ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. |
Uniprot ID | P18074 |
Protein Accession # | NP_000391 |
Nucleotide Accession # | NM_000400 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ERCC2 Antibody (OACA07226)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "ERCC2 Antibody (OACA07226)"?
This item is available "Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks".
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What buffer format is "ERCC2 Antibody (OACA07226)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ERCC2 Antibody (OACA07226)"?
This target may also be called "basic transcription factor 2 80 kDa subunit;BTF2 p80;COFS2;CXPD;DNA excision repair protein ERCC-2;DNA repair protein complementing XP-D cells;EM9;excision repair cross-complementation group 2;excision repair cross-complementing rodent repair deficiency, complementation group 2;general transcription and DNA repair factor IIH helicase subunit XPD;TFIIH;TFIIH 80 kDa subunit;TFIIH basal transcription factor complex 80 kDa subunit;TFIIH basal transcription factor complex helicase subunit;TFIIH basal transcription factor complex helicase XPB subunit;TFIIH basal transcription factor complex helicase XPD subunit;TFIIH p80;TFIIH subunit XPD;TTD;TTD1;xeroderma pigmentosum complementary group D;xeroderma pigmentosum group D-complementing protein;XPD." in publications.
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What is the shipping cost for "ERCC2 Antibody (OACA07226)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ERCC2 Antibody (OACA07226)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ERCC2 Antibody (OACA07226)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ERCC2 Antibody (OACA07226)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ERCC2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ERCC2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ERCC2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ERCC2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ERCC2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ERCC2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.