ERCC2 Antibody - middle region (OAGA02524)

Catalog No: OAGA02524

Size:100 ul

Sample (30 ug of whole cell lysate)
A: IMR32
B: U87-MG
7.5% SDS PAGE
OAGA02524 diluted at 1:1000

ERCC2 antibody [N2C2], Internal detects ERCC2 protein at cytosol and nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
ERCC2 antibody [N2C2], Internal (OAGA02524) dilution: 1:500.

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using ERCC2 (OAGA02524) antibody at 1:500 dilution.

ERCC2 antibody [N2C2], Internal detects ERCC2 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERCC2 protein stained by ERCC2 antibody [N2C2], Internal (OAGA02524) diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 1X-PBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human XPD. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Rat, Chimpanzee, Bovine
• Concentration: 1mg/ml
• Intended Use: For in vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
• Application Info: IF: 1:100~1000
  IHC-P: 1:100~1000
  WB: 1:500~3000
  

Target Info

• Gene Symbol: ERCC2
• Gene Full Name: excision repair cross-complementation group 2
• Alias Symbols: EM9, TTD, XPD, TTD1, COFS2, TFIIH
• NCBI Gene Id: 2068
• Description of Target: The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P18074
• Molecular Weight: 87 kDa