Catalog No: OKEH01334
Size:96T
Price: $525.00
SKU
OKEH01334
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

ERCC1 ELISA Kit (Human) : 96 Wells (OKEH01334)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ERCC1 ELISA Kit (Human) (OKEH01334) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERCC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ERCC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERCC1 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
ERCC1 Microplate96 Wells (12 x 8 Well strips)
ERCC1 Lyophilized Standard2
100X Biotinylated ERCC1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human DNA excision repair protein ERCC-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolERCC1
Gene Full NameERCC excision repair 1, endonuclease non-catalytic subunit
Alias SymbolsCOFS4;DNA excision repair protein ERCC-1;excision repair cross-complementation group 1;excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence);RAD10;UV20.
NCBI Gene Id2067
Protein NameP07992
Description of TargetDNA excision repair protein ERCC-1
Uniprot IDhttps://www.uniprot.org/uniprot/P07992
Protein Accession #NP_001159521.1
Nucleotide Accession #NM_001166049.1
Protein Size (# AA)297
Molecular Weight33 kDa
Write Your Own Review
You're reviewing:ERCC1 ELISA Kit (Human) : 96 Wells (OKEH01334)
Your Rating
We found other products you might like!