ERCC1 Antibody (OAAF01786)

Catalog No: OAAF01786 (Formerly GWB-ASD061)

Size:100 ug

Western blot analysis of extracts from NIH-3T3 cells, using ERCC1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ERCC1 Antibody. The picture on the right is blocked with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human ERCC1.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Concentration: 1 mg/ml
• Specificity: ERCC1 Antibody detects endogenous levels of total ERCC1 protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1,000
  IHC: 1:50~1:100
  ELISA: 1:5,000
  Optimal dilutions should be determined by the end user.
• Reference: Kan-Tai Hsia, Development, Jan 2003; 130: 369.
  Jim Selfridge, Nucleic Acids Res., Nov 2001; 29: 4541 - 4550.
  Cha-Kyung Youn, Cancer Res., Jul 2004; 64: 4849 - 4857.
  Teresa A. Motycka, J. Biol. Chem., Apr 2004; 279: 13634 - 13639.

Target Info

• Gene Symbol: ERCC1
• Gene Full Name: ERCC excision repair 1, endonuclease non-catalytic subunit
• Alias Symbols: COFS4;DNA excision repair protein ERCC-1;excision repair cross-complementation group 1;excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence);RAD10;UV20.
• NCBI Gene Id: 2067
• Protein Name: DNA excision repair protein ERCC-1
• Description of Target: Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
• Uniprot ID: P07992
• Protein Accession #: NP_001159521.1
• Molecular Weight: 32 kDa