ERBB3 ELISA Kit (Human) (OKCD01202)

Catalog No: OKCD01202 (Formerly GWB-KBBTN8)

Size:96WELLS

Human ERBB3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	83-95%	88-96%	78-99%	89-102%
  EDTA plasma(n=5)	78-103%	88-104%	91-98%	86-101%
  heparin plasma(n=5)	91-98%	78-94%	84-99%	91-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-104	101
  EDTA plasma(n=5)	79-104	88
  heparin plasma(n=5)	96-104	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ERBB3 Microplate: 96 Wells (12 x 8 Well Strips)
  • ERBB3 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated ERBB3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 32 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3). No significant cross-reactivity or interference between V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ERBB3
• Gene Full Name: erb-b2 receptor tyrosine kinase 3
• Alias Symbols: c-erbB3;c-erbB-3;ErbB-3;erbB3-S;FERLK;HER3;human epidermal growth factor receptor 3;LCCS2;MDA-BF-1;p180-ErbB3;p45-sErbB3;p85-sErbB3;proto-oncogene-like protein c-ErbB-3;receptor tyrosine-protein kinase erbB-3;tyrosine kinase-type cell surface receptor HER3;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3;VSCN1.
• NCBI Gene Id: 2065
• Protein Name: Receptor tyrosine-protein kinase erbB-3
• Description of Target: Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134).
• Uniprot ID: P21860
• Protein Accession #: NP_001005915
• Nucleotide Accession #: NM_001005915.1
• Protein Size (# AA): 1342