Catalog No: OKCD01155 (Formerly GWB-KBBOO2)
Size:96WELLS
Price: $700.00
SKU
OKCD01155
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Datasheets/ManualsPrintable datasheet for ERBB2 ELISA Kit (Rat) (OKCD01155)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)95-102%86-101%97-105%95-103%
EDTA plasma(n=5)85-101%95-102%88-96%98-105%
heparin plasma(n=5)92-105%78-93%88-95%92-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Tyrosine Protein Kinase erbB-2 (ErbB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)96-105101
EDTA plasma(n=5)91-9996
heparin plasma(n=5)88-10195
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Erbb2 Microplate96 Wells (12 x 8 Well Strips)
Erbb2 Lyophilized Standard2 x 40 ng
100X Biotinylated Erbb2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.221 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor Receptor 2 (EGFR2). No significant cross-reactivity or interference between Epidermal Growth Factor Receptor 2 (EGFR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolErbb2
Gene Full Nameerb-b2 receptor tyrosine kinase 2
Alias SymbolsAvian erythroblastosis viral (v-erb-B2) oncogene homologue 2 (neuro/glioblastoma derived oncogene homolog);C-erbB-2;epidermal growth factor receptor-related protein;HER2/neu;neu;NEU proto-oncogene;p185erbB2;p185neu;proto-oncogene c-ErbB-2;proto-oncogene NEU;receptor tyrosine-protein kinase erbB-2;receptor tyrosine-protein kinase erbB-2-like;v-erb-b2 avian erythroblastic leukemia viral oncogene 2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog.
NCBI Gene Id24337
Protein NameReceptor protein-tyrosine kinase
Description of TargetReceptor protein-tyrosine kinase
Uniprot IDQ8K3F9
Protein Accession #NP_058699
Nucleotide Accession #NM_017003.2
Protein Size (# AA)1259
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