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Catalog No: OKCD07745
Size:96WELLS
Price: $750.00
SKU
OKCD07745
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Datasheets/ManualsPrintable datasheet for ERBB2 ELISA Kit (Human) (OKCD07745)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Tyrosine Protein Kinase erbB-2 (ErbB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Tyrosine Protein Kinase erbB-2 (ErbB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ERBB2 Microplate96 Wells (12 x 8 Well strips)
ERBB2 Lyophilized Standard2
100X ERBB2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.229ng/mL
Gene SymbolERBB2
Gene Full Nameerb-b2 receptor tyrosine kinase 2
Alias SymbolsCD340;c-erb B2/neu protein;HER2;HER-2;HER-2/neu;herstatin;human epidermal growth factor receptor 2;metastatic lymph node gene 19 protein;MLN 19;NEU;neuro/glioblastoma derived oncogene homolog;neuroblastoma/glioblastoma derived oncogene homolog;NGL;p185erbB2;proto-oncogene c-ErbB-2;proto-oncogene Neu;receptor tyrosine-protein kinase erbB-2;TKR1;tyrosine kinase-type cell surface receptor HER2;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2;v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2;v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog;VSCN2.
NCBI Gene Id2064
Protein NameReceptor tyrosine-protein kinase erbB-2
Description of TargetProtein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
Uniprot IDP04626
Protein Accession #NP_001005862.1
Nucleotide Accession #NM_001005862.2
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