ERBB2 Antibody (Phospho-Tyr1223) (OAAB16277)

Catalog No: OAAB16277

Size:400ul

Dot blot analysis of Phospho-mouse ERBB2-Y1223 Antibody Phospho-specific Pab (Cat. # OAAB16277) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: DB
• Additional Information: Precautions: Phospho-mouse ERBB2-Y1223 is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity)
  Cellular Location: Cell membrane; Single-pass type I membrane protein (By similarity). Cytoplasm, perinuclear region (By similarity). Nucleus (By similarity)
  Tissue Location: Expressed predominantly in uterine epithelial cells. In the muscle, expression localizes to the synaptic sites of muscle fibers
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This mouse ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1223 of mouse ERBB2.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: DB: 1:500

Target Info

• Gene Symbol: Erbb2
• Alias Symbols: Ne, HER, Neu, HER-, HER2, c-ne, ErbB-, HER-2, c-neu, l11Ju, Erbb-2, c-erbB, c-erbB2, l11Jus8, mKIAA3023
• NCBI Gene Id: 13866
• Protein Name: Receptor tyrosine-protein kinase erbB-2
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P70424
• Protein Accession #: NP_001003817.1
• Molecular Weight: 138579 Da