ERAP2 ELISA Kit (Human) (OKCD00998)

Catalog No: OKCD00998 (Formerly GWB-KBBPF5)

Size:96WELLS

Human ERAP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endoplasmic Reticulum Aminopeptidase 2 (ERAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endoplasmic Reticulum Aminopeptidase 2 (ERAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endoplasmic Reticulum Aminopeptidase 2 (ERAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ERAP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • ERAP2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated ERAP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.128 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Endoplasmic Reticulum Aminopeptidase 2 (ERAP2). No significant cross-reactivity or interference between Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ERAP2
• Gene Full Name: endoplasmic reticulum aminopeptidase 2
• Alias Symbols: endoplasmic reticulum aminopeptidase 2;leukocyte-derived arginine aminopeptidase;LRAP;L-RAP.
• NCBI Gene Id: 64167
• Protein Name: Endoplasmic reticulum aminopeptidase 2
• Description of Target: Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Preferentially hydrolyzes the basic residues Arg and Lys.
• Uniprot ID: Q6P179
• Protein Accession #: NP_001123612
• Nucleotide Accession #: NM_001130140.1
• Protein Size (# AA): 960