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| Datasheets/Manuals | Printable datasheet for ER-ALPHA Antibody (OAAD00234) - OAAD00234 |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Clonality | Monoclonal |
| Clone | 2B7 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB, IHC |
| Reconstitution and Storage | Store at 4C, for long term storage, store at -20C. |
| Immunogen | Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli. |
| Formulation | Purified antibody in PBS containing 0.03% sodium azide. |
| Application Info | Western Bloting: 1/500 - 1/2000. Immunohistochemistry: 1/200 - 1/1000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test. |
| Reference | 1. Paech K. Science 1997, 277: 1508- 10. 2. Pertschuk LP£¬Cancer, 1996. 199677: 514- 9. |
| Gene Symbol | ER-Alpha |
|---|---|
| Alias Symbols | ER, ESR, Era, ESRA, ESTRR, NR3A1 |
| NCBI Gene Id | 2099 |
| Description of Target | The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells. |
| Uniprot ID | P03372 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ER-ALPHA Antibody (OAAD00234)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "ER-ALPHA Antibody (OAAD00234)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "ER-ALPHA Antibody (OAAD00234)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ER-ALPHA Antibody (OAAD00234)"?
This target may also be called "ER, ESR, Era, ESRA, ESTRR, NR3A1" in publications.
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What is the shipping cost for "ER-ALPHA Antibody (OAAD00234)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ER-ALPHA Antibody (OAAD00234)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ER-ALPHA Antibody (OAAD00234)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ER-ALPHA Antibody (OAAD00234)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ER-ALPHA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ER-ALPHA"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ER-ALPHA"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ER-ALPHA"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ER-ALPHA"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ER-ALPHA"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
