EPX ELISA Kit (Mouse) (OKCA02171)

Catalog No: OKCA02171

Size:96 Wells

Mouse EPX ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	88	84-92
  Serum (n=4)	1:2	94	90-98
  Serum (n=4)	1:4	99	95-104
  Serum (n=4)	1:8	102	98-107

• ELISA Kit Principle: Aviva Systems Biology Epx ELISA Kit (Mouse) (OKCA02171) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EPX captured in well.
• ELISA Kit Range: 0.156 ng/mL-10 ng/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	95	92-98
  EDTA plasma (n=4)	96	94-98

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-EPX Microplate: 96 Wells (12 x 8 Well strips)
  • EPX Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated EPX Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.039 ng/mL.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EPX
• Alias Symbols: E, EPO
• NCBI Gene Id: 13861
• Protein Name: Eosinophil peroxidase
• Description of Target: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils.
• Uniprot ID: P49290
• Protein Accession #: NP_031972
• Nucleotide Accession #: NM_007946