Size:96 Wells
$480.00
Discontinued
SKU
OKCA00188
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Epidemic Hemorrhagic Fever Virus Antibody (IgG) ELISA Kit (Rat) (OKCA00188)

Catalog#: OKCA00188
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Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration < 4 Hours
Kit Principle Aviva Systems Biology EHF Antibody (IgG) ELISA Kit (Rat) (OKCA00188) is based on a qualitative reverse phase enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with EHF antigen and blocked. Samples or controls are added to the plate wells, incubated and washed. A horseradish peroxidase (HRP) conjugated anti-Rat IgG detector antibody is then added, incubated and washed. EHF Antibody in samples or controls will bind to the antigen immobilized in the well and are subsequently bound by the HRP conjugated anti-Rat IgG antibody. Washing removes unbound sample and detector antibody. An enzymatic reaction is then produced through the addition of reporter substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is proportional to the amount of EHF Antibody contained in the sample or standard.
Kit Reproducibility

Mean Intra-Assay Precision %CV: < 15% (n = 20)

Mean Inter-Assay Precision %CV: < 15% (n = 20)

Kit Component
ComponentAmount
EHF Microplate96 Well (12 x 8 Well Strips)
Negative Control1 x 500 uL
Positive Control1 x 500 uL
Sample Diluent2 x 20 mL
Anti-Rat IgG Antibody HRP-Conjugate1 x 12 mL
20X Wash Buffer1 x 7 mL
Substrate A1 x 7 mL
Substrate B1 x 7 mL
Stop Solution1 x 7 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of rat epidemic hemorrhagic fever antibody (IgG). No significant cross-reactivity or interference between rat epidemic hemorrhagic fever antibody (IgG) and
analogues was observed.
Assay Info Assay Methodology: Qualitative Reverse Capture Immunoassay
Gene Symbol EHF IgG
Alias Symbols EGF
Protein Name Epidemic Hemorrhagic Fever Virus Antibody (IgG)
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