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Catalog No: OKCD02524
Size:96WELLS
Price: $750.00
SKU
OKCD02524
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Datasheets/ManualsPrintable datasheet for EPHX4 ELISA Kit (Mouse) (OKCD02524)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)81-89%89-97%80-88%78-104%
EDTA Plasma (n=5)78-94%98-105%88-101%79-104%
Heparin Plasma (n=5)94-105%78-99%91-105%83-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epoxide Hydrolase 4 (EPHX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epoxide Hydrolase 4 (EPHX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epoxide Hydrolase 4 (EPHX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Epoxide Hydrolase 4 (EPHX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range62.5-4,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)92-10595
EDTA Plasma (n=5)88-10297
Heparin Plasma(n=5)87-9590
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epoxide Hydrolase 4 (EPHX4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epoxide Hydrolase 4 (EPHX4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-EPHX4 Microplate96 Wells (12 x 8 Well strips)
EPHX4 Lyophilized Standard2
100X Biotinylated EPHX4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity27.2 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Epoxide Hydrolase 4 (EPHX4). No significant cross-reactivity or interference between Epoxide Hydrolase 4 (EPHX4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolEphx4
Gene Full Nameepoxide hydrolase 4
Alias SymbolsAbhd;Abhd7;abhydrolase domain containing 7;abhydrolase domain-containing protein 7;Ephxrp;epoxide hydrolase 4;epoxide hydrolase-related protein;Gm1382.
NCBI Gene Id384214
Protein NameEpoxide hydrolase 4
Description of TargetEpoxide hydrolase 4
Uniprot IDQ6IE26
Protein Accession #NP_001001804.2
Nucleotide Accession #NM_001001804.2
Protein Size (# AA)359
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