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Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)
| Datasheets/Manuals | Printable datasheet for OAAF00520 |
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| Predicted Species Reactivity | Human, Mouse, Rat |
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| Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB, IHC |
| Additional Information | Target Modification: Phospho Modification Sites: H:Y324/311/318 M:Y323/314/318 R:Y323/-/318 |
| Reconstitution and Storage | Store at -20C/1 year |
| Immunogen | The antiserum was produced against synthesized peptide derived between 290-339 amino acids of human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Specificity | Ephrin B1/B2/B3 (Phospho-Tyr324) antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324. |
| Dilution | The optimal working dilution should be determined by the end user. |
| Application Info | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
| Reference | Beckmann M.P., EMBO J. 13:3757-3762(1994). Davis S., Science 266:816-819(1994). Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases. |
| Publications | Activating Mutations in PIK3CA Lead to Widespread Modulation of the Tyrosine Phosphoproteome. J. Proteome Res. 14, 3882-91 (2015). 26267517 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
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How long will it take to receive "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)"?
This target may also be called "HTKL, EPLG5, Htk-L, LERK5" in publications.
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What is the shipping cost for "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "38 kD".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EFNB3, EFNB1, EFNB2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EFNB3, EFNB1, EFNB2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EFNB3, EFNB1, EFNB2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EFNB3, EFNB1, EFNB2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EFNB3, EFNB1, EFNB2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EFNB3, EFNB1, EFNB2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.