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Conjugation Options

OAAF00520-FITC Conjugated

OAAF00520-HRP Conjugated

OAAF00520-Biotin Conjugated

Ephrin B1/B2/B3 Antibody (Phospho-Tyr324) (OAAF00520)

Catalog#: OAAF00520
Domestic: within 1 week delivery International: 1 week
This product is Genway GWB-ASB962
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More Information
Predicted Species Reactivity Human, Mouse, Rat
Product Format Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality Polyclonal
Isotype IgG
Host Rabbit
Application WB, IHC
Additional Information Target Modification: Phospho
Modification Sites: H:Y324/311/318 M:Y323/314/318 R:Y323/-/318
Reconstitution and Storage Store at -20C/1 year
Immunogen The antiserum was produced against synthesized peptide derived between 290-339 amino acids of human Ephrin B1/B2/B3 around the phosphorylation site of Tyr324.
Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Datasheets/Manuals Printable datasheet for OAAF00520
Specificity Ephrin B1/B2/B3 (Phospho-Tyr324) antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324.
Dilution The optimal working dilution should be determined by the end user.
Application Info WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:20000
Target Reference Beckmann M.P., EMBO J. 13:3757-3762(1994).
Davis S., Science 266:816-819(1994).
Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.

Zahari, MS; Wu, X; Blair, BG; Pinto, SM; Nirujogi, RS; Jelinek, CA; Malhotra, R; Kim, MS; Park, BH; Pandey, A; Activating Mutations in PIK3CA Lead to Widespread Modulation of the Tyrosine Phosphoproteome. 14, 3882-91 (2015). WB, IHC, ELISA, Human, Mouse, Rat 26267517

Official Gene Full Name Ephrin-B1, Ephrin-B2, Ephrin-B3
Target EFNB3, EFNB1, EFNB2
Alias Symbols EPLG5, HTKL, LERK5, EFNB1, EFNB2, EFNB3
NCBI Gene Id 1948
Protein Name Ephrin-B1, Ephrin-B2, Ephrin-B3
Swissprot Id P98172, P52799, Q15768
Protein Accession # NP_001397.1
Molecular Weight 38 kD
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express EFNB2.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express EFNB2.
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