EPHB1/2/3 Antibody (OAAF03647)

Catalog No: OAAF03647 (Formerly GWB-ASE584)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EPHB1/2/3 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using EPHB1/2/3 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human EPHB1/2/3.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: EPHB1/2/3 antibody detects endogenous levels of total EPHB1/2/3 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
• Reference: Tang X.X., Genomics 29:426-437(1995).
  Zhang Z., Protein Sci. 13:2819-2824(2004).
  Stein E., J. Biol. Chem. 271:23588-23593(1996).

Target Info

• Gene Symbol: EPHB1|EPHB2|EPHB3
• Gene Full Name: EPH receptor B1|EPH receptor B2|EPH receptor B3
• Alias Symbols: BDPLT22;CAPB;developmentally-regulated Eph-related tyrosine kinase;DRT;EK2;EK5;EK6;ELK;elk-related tyrosine kinase;embryonic kinase 2;eph tyrosine kinase 2;eph tyrosine kinase 3;EPH-like kinase 5;EPH-like kinase 6;EPH-like tyrosine kinase 2;ephrin type-B receptor 1;ephrin type-B receptor 2;ephrin type-B receptor 3;EPHT2;EPHT3;ERK;ETK2;HEK2;Hek5;Hek6;human embryo kinase 2;NET;neuronally-expressed EPH-related tyrosine kinase;PCBC;protein-tyrosine kinase HEK5;renal carcinoma antigen NY-REN-47;soluble EPHB1 variant 1;Tyro5;TYRO6;tyrosine-protein kinase receptor EPH-2;tyrosine-protein kinase receptor EPH-3;tyrosine-protein kinase TYRO5;tyrosine-protein kinase TYRO6.
• NCBI Gene Id: 2047|2048|2049
• Protein Name: Ephrin type-B receptor 1|Ephrin type-B receptor 2|Ephrin type-B receptor 3
• Description of Target: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity).|Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor.|Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
• Uniprot ID: P29323|P54753|P54762
• Protein Accession #: NP_004434.2
• Molecular Weight: 109 kDa